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Research

The work in our lab involves insects, plants, and the interactions between them. We use methods ranging from field ecology to genomics to tackle interesting questions.

Current projects include work on the evolution of lodgepole and jack pine chemical defenses against insects and pathogens, olfaction and foraging behavior in bark beetles, cold tolerance of bark beetles, detoxification of host resin compounds by bark beetles, development of feeding deterrents for stored product pests, pheromone evolution in bark beetles, aquatic insect biodiversity, and other entomological and chemical ecological research.

If you are interested in pursuing a project along similar lines, please contact me, and we can discuss it further.

Please take a look at the list of publications below for a taste of what we do in our lab.

An up-to-date list with links to find these articles and others that cite them, can be found here.

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Pitt, C., Robert, J.A., Bonnett, T.R., Keeling, C.I., Bohlmann, J., Huber, D.P.W. 201X. Proteomics indicators of the rapidly shifting physiology of mountain pine beetle, Dendroctonus ponderosae (Coleoptera: Curculionidae), adults during early host colonization. PLOS ONE (accepted 17 September 2014).

McKee, F.R., Huber, D.P.W.,  Lindgren, B.S., Hodgkinson, R.S., Aukema B.H. 2014. Effect of natal and colonised host species on female host acceptance and male joining behaviour of the mountain pine beetle (Coleoptera: Curculionidae) using pine and spruce . The Canadian Entomologist: doi:10.4039/tce.2014.22.

Janes, J.K., Li, Y., Keeling, C.I., Yuen, M.M.S., Boone, C.K., Cooke, J.E.K., Bohlmann, J., Huber, D.P.W., Murray, B.W., Coltman, D.W., Sperling, F.A.H. 201X. How the mountain pine beetle (Dendroctonus ponderosae) breached the Canadian Rocky Mountains. Molecular Biology and Evolution in press. OPEN ACCESS LINK

Clark, E.L., Pitt, C., Carroll, A.L., Lindgren, B.S., Huber, D.P.W. 2014. Comparison of lodgepole and jack pine resin chemistry: implications for range expansion by the mountain pine beetle, Dendroctonus ponderosae (Coleoptera: Curculionidae). PeerJ 2:e240. OPEN ACCESS LINK

Clark, E.L., Isitt, R., Plettner, E., Fields, P.G., Huber, D.P.W. 2014. An inexpensive feeding bioassay technique for stored-product insects. Journal of Economic Entomology 107:455-461. OPEN ACCESS LINK

Robert, J.A., Pitt, C., Bonnett, T.R., Yuen, M.M.S., Keeling, C.I., Bohlmann, J., and Huber, D.P.W. 2013. Disentangling detoxification: Gene expression analysis of feeding mountain pine beetle illuminates molecular-level host chemical defense detoxification mechanisms. PLoS ONE 8(11): e77777. OPEN ACCESS LINK

Gregory, T.R., Nathwani, P., Bonnett, T.R., and Huber, D.P.W. 2013. Sizing up arthropod genomes: an evaluation of the impact of environmental variation on genome size estimates by flow cytometry and the use of qPCR as a method of estimation. Genome 56:505-510. LINK

McKee, F.R., Huber, D.P.W, Aukema, B.H. 2013. Comparisons of mountain pine beetle (Dendroctonus ponderosae Hopkins) reproduction within a novel and traditional host: effects of insect natal history, colonized host species, and competitors. Agricultural and Forest Entomology 15:310-320. LINK

Keeling, C.I., Yuen, M.M.S., Liao N.Y., Docking, T.R., Chan, S.K., Taylor, G.A., Palmquist, D.L., Jackman, S.D., Nguyen, A., Li, M., Henderson, H., Janes, J.K., Zhao, Y., Pandoh, P., Moore, R., Sperling, F.A.H., Huber, D.P.W., Birol, I., Jones, S.J.M., Bohlmann, J. 2013. Draft genome of the mountain pine beetle, Dendroctonus ponderosae Hopkins, a major forest pest. Genome biology 14.3: R27. OPEN ACCESS LINK

Bonnett, T.R., Robert, J.A., Pitt, C., Fraser, J.D., Keeling, C.I., Bohlmann, J., Huber, D.P.W. 2012. Global and comparative proteomic profiling of overwintering and developing mountain pine beetle, Dendroctonus ponderosae (Coleoptera: Curculionidae), larvae. Insect Biochemistry and Molecular Biology 42:890-901. OPEN ACCESS LINK

Fettig, C.J., McKelvey, S.R., Dabney, C.P., Fowler, D., Huber, D.P.W., Lait, C., Borden, J.H. 2012. Efficacy of “Verbenone Plus” for protecting ponderosa pine trees and stands from Dendroctonus brevicomis (Coleoptera: Curculionidae) attack in British Columbia and California. Journal of Economic Entomology 105:1477-1870. LINK

Keeling, C.I., Henderson, H., Li, M., Yuen, M., Clark, E.L., Fraser, J.D., Huber, D.P.W., Liao, N.Y., Docking, T.R., Birol, I., Chan, S.K., Taylor, G.A., Palmquist, D., Jones, S.J.M., Bohlmann, J. 2012. Transcriptome and full-length cDNA resources for the mountain pine beetle, Dendroctonus ponderosae Hopkins, a major insect pest of pine forests. Insect Biochemistry and Molecular Biology 42: 525-536. LINK

Clark, E.L., Huber, D.P.W., and Carroll, A.L. 2012. The legacy of attack: implications of high phloem resin monoterpene levels in lodgepole pines following mass attack by mountain pine beetle, Dendroctonus ponderosae Hopkins. Environmental Entomology 41: 392-398. LINK

Fettig, C.J., McKelvey, S.R., Dabney, C.P., and Huber, D.P.W. 2012. Responses of Dendroctonus brevicomis (Coleoptera: Curculionidae) in behavioral assays: implications to development of a semiochemical-based tool for tree protection. Journal of Economic Entomology 105: 149-160. LINK

Ott, D.S., Yanchuk, A.D., Huber, D.P.W., and Wallin, K.F. 2011. Genetic variation of lodgepole pine, Pinus contorta var. latifolia, chemical and physical defenses that affect mountain pine beetle, Dendroctonus ponderosae, attack and tree mortality. Journal of Chemical Ecology 37: 1002-1012. LINK

Wallis, C.M., Huber, D.P.W., and Lewis, K.J. 2011 Ecosystem, location, and climate effects on foliar secondary metabolites of lodgepole pine populations from central British Columbia. Journal of Chemical Ecology, 37: 607-621. LINK

Wallis, C.M., Reich, R.W., Lewis, K.J., and Huber, D.P.W. 2010. Lodgepole pine provenances differ in chemical defense capacities against foliage and stem diseases. Canadian Journal of Forest Research 40: 2333-2344. LINK

Clark, E.L., Carroll, A.C., and Huber, D.P.W. 2010. Differences in lodgepole pine constitutive terpene profile across a geographic range in British Columbia and the correlation to historical attack by mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae). The Canadian Entomologist 142: 557-573. LINK

Fettig, C.J., McKelvey, S.R., Dabney, C.P., Borys, R.R., and Huber, D.P.W. 2009. Response of Dendroctonus brevicomis to different release rates of nonhost angiosperm volatiles and verbenone in trapping and tree protection studies. Journal of Applied Entomology 133: 143-154. LINK

Huber, D.P.W., Aukema, B.H., Hodgkinson, R.W., and Lindgren B.S. 2009. Successful colonization, reproduction, and new generation emergence in live interior hybrid spruce, Picea engelmannii x glauca, by mountain pine beetle, Dendroctonus ponderosae. Agricultural and Forest Entomology 11:83 – 89. LINK

Graves, A.D., Holsten, E.H., Ascerno, M.E., Zogas, K., Hard, J.S., Huber, D.P.W., Blanchette, R., and Seybold, S.J. 2008. Protection of spruce from colonization by the bark beetle, Ips perturbatus, in Alaska. Forest Ecology and Management 256:1825 – 1839. LINK

Fettig, C.J., Dabney, C.P., McKelvey, S.R., and Huber, D.P.W. 2008. Nonhost angiosperm volatiles and verbenone protect individual ponderosa pines from attack by western pine beetle and red turpentine beetle (Coleoptera: Curculionidae, Scolytinae). Western Journal of Applied Forestry 23:40 – 45. LINK

Shepherd, W.P., Huber, D.P.W., Seybold, S.J., and Fettig, C.J. 2007. Antennal responses of the western pine beetle, Dendroctonus brevicomis (Coleoptera: Curculionidae), to stem volatiles of its primary host, Pinus ponderosa, and nine sympatric nonhost angiosperms and conifers. Chemoecology 17:209 – 221. LINK

Huber, D.P.W., Erickson, M.L., Leutenegger, C.W., Bohlmann, J., and Seybold, S.J. 2007. Isolation and extreme sex-specific expression of cytochrome P450 genes in the bark beetle, Ips paraconfusus, following feeding on the phloem of host ponderosa pine, Pinus ponderosa. Insect Molecular Biology, 16:335 – 349. LINK

Ralph, S., Oddy C., Cooper, D., Yueh, H., Jancsik, S., Kolosova, N., Philippe, R.N., Aeschliman, D., White, R., Huber, D., Ritland, C.E., Benoit, F., Rigby, T., Nantel, A., Butterfield, Y.S.N., Kirkpatrick, R., Chun, E., Liu, J., Palmquist, D., Wynhoven, B., Stott, J., Yang, G., Barber, S., Holt, R.A., Siddiqui, A., Jones, S.J.M., Marra, M.A., Ellis, B.E., Douglas, C.J., Ritland, K., Bohlmann, J. 2006. Genomics of hybrid poplar (Populus trichocarpa x deltoides) interacting with forest tent caterpillars (Malacosoma disstria): Normalized and full-length cDNA libraries, expressed sequence tags (ESTs), and a cDNA microarray for the study of insect-induced defenses in poplar. Molecular Ecology 15:1275 – 1297. LINK

Seybold, S.J., Huber, D.P.W., Lee, J.C., Graves, A.D., and Bohlmann, J. 2006. Pine monoterpenes and pine bark beetles: a marriage of convenience for defense and chemical communication. Phytochemistry Reviews 5:143 – 178. LINK

Fettig, C.J., McKelvey, S.R., and Huber, D.P.W. 2005. Nonhost angiosperm volatiles and verbenone disrupt response of western pine beetle, Dendroctonus brevicomis (Coleoptera: Scolytidae), to attractant-baited traps. Journal of Economic Entomology 98:2041 – 2048. LINK

Huber, D.P.W., Philippe, R.N., Goddard, K-A., Sturrock, R.N., and Bohlmann, J. 2005. Characterization of four terpene synthases from a methyl jasmonate-induced Douglas-fir, Pseudotsuga menziesii (Mirbel) Franco (Pinaceae), cDNA library. Phytochemistry 66:1427 – 1439. LINK

Huber, D.P.W., Philippe, R.N., Madilao, L., Sturrock, R.N., and Bohlmann, J. 2005. Changes in anatomy and terpene chemistry in Douglas-fir, Pseudotsuga menziesii (Mirbel) Franco, seedlings following treatment of roots with methyl jasmonate. Tree Physiology 25:1075 – 1083. LINK

Huber, D.P.W., Ralph, S., and Bohlmann, J.2004. Genomic hardwiring and phenotypic plasticity of terpenoid based defenses in conifers. Journal of Chemical Ecology 30:2399 – 2418. LINK

Huber, D.P.W.and Bohlmann, J. 2005. The role of terpene synthases in the direct and indirect defense of conifers against insect herbivory and fungal pathogens. In: Multigenic and Induced Systemic Resistance in Plants. S.Tuzun (ed.). Kluwer Academic Press, Dordrecht, the Netherlands. pp. 297 – 313. LINK

Bohlmann, J., Martin, D., Miller, B., and Huber, D.P.W. 2004. Terpenoid synthases in conifers and poplars. In: Plantation Forest Biotechnology for the 21st Century. C. Walter and M. Carson (eds.). Research Signpost, Kerala, India. pp. 181 – 201. LINK

Arimura, G-I., Huber, D.P.W., and Bohlmann, J. 2004. Forest tent caterpillars (Malacosoma disstria) induce local and systemic diurnal emissions of terpenoid volatiles in hybrid poplar (Populus trichocarpa x deltoides): cDNA cloning, functional characterization, and patterns of gene expression of (-)-germacrene D synthase, PtdTPS1. The Plant Journal 37: 603-616. LINK

Huber, D.P.W., and Bohlmann, J. 2004. Terpene synthases and the mediation of plant-insect ecological interactions by terpenoids: a mini-review. In: Plant Adaptation: Molecular Genetics and Ecology. Q.C.B. Cronk, J. Whitton, R.H. Ree, and I.E.P. Taylor (eds.). NRC Research Press, Ottawa, Ontario. pp. 70-81. LINK

Huber, D.P.W., and Borden, J.H. 2003. Comparative behavioural responses of Dryocoetes confusus Swaine, Dendroctonus rufipennis (Kirby), and Dendroctonus ponderosae Hopkins (Coleoptera: Scolytidae) to angiosperm tree bark volatiles. Environmental Entomology 32:742 – 751. LINK

Borden, J.H., Chong, L.J., Earle, T.J., and Huber, D.P.W. 2003. Protection of lodgepole pine from attack by the mountain pine beetle, Dendroctonus ponderosae (Coleoptera: Scolytidae) using high doses of verbenone in combination with nonhost bark volatiles. The Forestry Chronicle 79: 685 – 691. LINK

Huber, D.P.W., Borden, J.H. and Stastny, M. 2001. Response of the pine engraver, Ips pini (Say) (Coleoptera: Scolytidae), to conophthorin and other angiosperm bark volatiles in the avoidance of nonhosts. Agricultural and Forest Entomology 3: 225 – 232. LINK

Huber, D.P.W. and Borden, J.H. 2001. Protection of lodgepole pines from mass attack by mountain pine beetle, Dendroctonus ponderosae, with nonhost angiosperm volatiles and verbenone. Entomologia Experimentalis et Applicata 99:131 – 141. LINK

Huber, D.P.W. and Borden, J.H. 2001. Angiosperm bark volatiles disrupt response of Douglas-fir beetle, Dendroctonus pseudotsugae, to attractant-baited traps. Journal of Chemical Ecology 27: 217 – 233. LINK

Borden, J.H., Chong, L.J., Gries, G., Gries, R., Huber, D.P.W., Pierce, H.D., Jr., and Wilson, I.M. 2001. Nonhost volatiles as repellents for conifer-infesting bark beetles. US Patent # 6217891. LINK

Huber, D.P.W., Borden, J.H., Gries, R., and Jeans-Williams N.L. 2000. Differential bioactivity of conophthorin on four species of North American bark beetles (Coleoptera: Scolytidae). The Canadian Entomologist 132: 649 – 653. LINK

Huber, D.P.W., Gries, R., Borden, J.H., and Pierce, H.D., Jr. 2000. A survey of antennal responses by five species of coniferophagous bark beetles (Coleoptera: Scolytidae) to bark volatiles of six species of angiosperm trees. Chemoecology 10:103 – 113. LINK

Borden, J.H., Chong, L.J., Gries, G., Gries, R., Huber, D.P.W., Pierce, H.D., Jr., and Wilson, I.M. 2000. Nonhost volatiles as repellents for conifer-infesting bark beetles. US Patent # 6051612. LINK

Huber, D.P.W., Gries, R., Borden, J.H., and Pierce, H.D., Jr. 1999. Two pheromones of coniferophagous bark beetles found in the bark of non-host angiosperms. Journal of Chemical Ecology. 25: 805 – 816. LINK

Karkhoff-Schweizer, R.R., Huber, D.P.W., and Voordouw, G. 1995. Conservation of the genes for dissimilatory sulfite reductase from Desulfovibrio vulgaris and Archaeoglobus fulgidus allows their detection by PCR. Applied and Environmental Microbiology 61:290 – 296. LINK